Whole Transcriptome Sequencing Package (Library Prep + Illumina PE150 Sequencing) with standard analysis. Select 12 Gb or 20 Gb PF data through Package Option.
Vendor Description by Package Option: AZ-WTS-12G — Basic BI Included; standard analysis for 12 Gb PF data. AZ-WTS-20G — Basic BI Included; standard analysis for 20 Gb PF data.
2 x libraries: 1 for transcripts and 1 for small RNA
Sample Requirement
≥ 4 μg, with a concentration of ≥ 200 ng/μL.
BIOINFORMATICS ANALYSIS (Standard)
Data QC & Genome Alignment — Raw-read quality control (Q20/Q30, GC, error-rate profiling), adapter and low-quality trimming, and alignment to the reference genome with mapping, genomic-distribution and saturation diagnostics.
1. Quantification & Normalization — Expression quantification and normalization (e.g. FPKM/read counts) for all detected RNA species: mRNA, lncRNA, circRNA and miRNA, including identification of novel lncRNAs and novel miRNAs.
2. Correlation Analysis — Sample-to-sample correlation and clustering to assess reproducibility between replicates and separation between experimental groups.
3. Differential Expression (DEG) Analysis — Differential-expression analysis between groups for each RNA class — differential mRNAs, lncRNAs, circRNAs and miRNAs.
4. Functional Annotation & Enrichment — GO and KEGG pathway enrichment of differentially expressed genes and predicted targets, with lncRNA/miRNA target-gene prediction to link non-coding regulators to biological function.
5. Advanced Regulatory & Structural Analysis — Alternative-splicing detection and annotation, SNP/SNV variant calling, transcript structural analysis, and ceRNA (miRNA-sponge) network construction linking circRNA/lncRNA–miRNA–mRNA interactions.
Deliverables include a fully documented report with publication-ready figures and tables, a results-directory readme, a detailed methods document, and processed data files.
