Bacterial or fungal sample QC, library preparation, and sequencing. The Package Option specifies platform, PF output, and whether standard analysis is included; standard analysis excludes species identification. For Illumina Standard BI options, legacy scope specifies total DNA ≥400 ng and ≥2 ng/µL, SNP/InDel detection and annotation at ≥100× depth (1–2 Gb bacteria, 2–4 Gb fungi recommended), and structural-variation detection and annotation at ≥200× depth (2–4 Gb bacteria, 4–8 Gb fungi recommended).
The selected Package Option governs. Only the entry matching its Darwin reference applies.
Vendor Description and Sample Requirement by Package Option:
Darwin references: AZ-MGR-ILL-NA-1G, AZ-MGR-ILL-NA-2G, AZ-MGR-ILL-NA-4G
Vendor Description: No BI
Sample Requirement: Total DNA ≥400 ng; DNA Concentration ≥2 ng/μL
Darwin references: AZ-MGR-ILL-SA-1G, AZ-MGR-ILL-SA-2G, AZ-MGR-ILL-SA-4G, AZ-MGR-MGI-SA-1G, AZ-MGR-MGI-SA-2G, AZ-MGR-MGI-SA-4G
Vendor Description: Basic BI Included: Standard Analysis
• SNP & InDel Detection/Annotation: ≥100X (Bacteria: 1–2 Gb recommended; Fungi: 2–4 Gb recommended).
• SV (Structural Variation) Detection/Annotation: ≥200X depth (Bacteria: 2–4 Gb recommended; Fungi: 4–8 Gb recommended).
• Excludes Species Identification
Sample Requirement: Total DNA ≥400 ng; DNA Concentration ≥2 ng/μL
