Sample QC, Agilent V6 exome capture library preparation, and 150PE sequencing. The Package Option specifies platform, 10/20/50 Gb output, FFPE handling where applicable, and standard analysis inclusion. For standard analysis, disease WES includes SNP/InDel/CNV (CNV requires >8 client control samples); cancer WES requires paired tumor-normal samples for germline/somatic SNV/InDel and somatic CNV.
The selected Package Option governs. Only the entry matching its Darwin reference applies.
Vendor Description and Sample Requirement by Package Option:
Darwin references: AZ-HWES-ILL-V6-NA-10G, AZ-HWES-ILL-V6-NA-20G, AZ-HWES-ILL-V6FFPE-NA-20G, AZ-HWES-ILL-V6-NA-50G, AZ-HWES-MGI-V6-NA-10G, AZ-HWES-MGI-V6-NA-20G, AZ-HWES-MGI-V6FFPE-NA-20G, AZ-HWES-MGI-V6-NA-50G
Vendor Description: No BI
Sample Requirement: Total DNA ≥500 ng; DNA Concentration ≥10 ng/μl
Darwin references: AZ-HWES-ILL-V6-SA-10G, AZ-HWES-ILL-V6-SA-20G, AZ-HWES-ILL-V6FFPE-SA-20G, AZ-HWES-ILL-V6-SA-50G, AZ-HWES-MGI-V6-SA-10G, AZ-HWES-MGI-V6-SA-20G, AZ-HWES-MGI-V6FFPE-SA-20G, AZ-HWES-MGI-V6-SA-50G
Vendor Description: Basic BI Included: Standard Analysis
• Disease WES: Includes SNP, InDel, and CNV analysis (Note: CNV analysis requires >8 control samples provided by the client).
• Cancer WES: Includes detection of Germline SNV/InDel, Somatic SNV/InDel, and Somatic CNV (Requires tumor-normal paired samples).
Sample Requirement: Total DNA ≥500 ng; DNA Concentration ≥10 ng/μl
