Reverse transcription is one of the most error-prone steps in any qPCR workflow. Multiple pipetting steps, reagent exposure, and the ever-present threat of RNase contamination all introduce variability that researchers do not need.
If you want a cleaner, more reproducible start to every expression experiment, NZY RT SuperMix 5× was engineered precisely for that purpose — and we are now offering limited sampler units for researchers who want to validate this premium Portugal-made mix in their own RT-qPCR workflow.
🔗 Request your sampler (while stocks last):
https://www.darwinscience.com/contactus
Why One-Tube RT Makes a Difference
Traditional RT setups require mixing multiple components — RT enzyme, buffer, dNTPs, RNase inhibitor, primers — each introducing more chances for pipetting error and contamination.
The NZY RT SuperMix 5× consolidates everything into one ready-to-use master mix that contains:
- RNase-H minus reverse transcriptase
- RNase inhibitor
- dNTPs
- Optimized RT buffer
- Random hexamers + oligo(dT)₁₈ priming system
- Stabilizers for consistent performance
This single-tube configuration dramatically reduces hands-on burden while protecting RNA integrity — especially important for delicate or low-abundance samples.
Fast, Precise, and Built for Modern qPCR Workflows
10-Minute RT Reaction
A complete first-strand cDNA synthesis is done in as fast as 10 minutes at 50–55 °C, making it ideal for high-throughput labs or rapid turnaround studies.
Higher RNA Input Flexibility
The 5× format allows you to add more RNA per reaction, supporting a wide input range from 10 pg to 5 µg.
This is particularly valuable for:
- Low-input clinical or environmental RNA
- Rare transcripts
- High-sensitivity gene expression assays
Consistent, Contamination-Resistant Performance
By reducing pipetting steps, you naturally minimize:
- Aerosol contamination risk
- Cross-sample contamination
- Variability between technicians
- RNase exposure
- Setup time
It’s a straightforward way to tighten workflow precision — without changing your qPCR pipeline.
Ideal for All RT-qPCR Applications
The NZY RT SuperMix 5× is validated for downstream use in:
- Real-time PCR
- Gene expression studies
- RNA quantification
- General PCR workflows
It is available in two pack sizes:
🔹 50 reactions
🔹 250 reactions
📦 Product page:
https://www.darwinscience.com/shop/nzy-rt-supermix-5x-548145#attr=1654
Sampling Campaign: Try It Before You Switch
We are running a sampling campaign from now until 24 March 2025, enabling researchers to test the NZY RT SuperMix 5× directly within their lab’s real-time PCR workflow.
If you value clean setup, reproducibility, and high-quality cDNA — this is the perfect opportunity to evaluate it firsthand.
👉 🔗 Request your sampler (while stocks last):
https://www.darwinscience.com/contactus
